ChIP — Does it work correctly? The optimization steps of chromatin immunoprecipitation
نویسندگان
چکیده
منابع مشابه
Chromatin immunoprecipitation (ChIP).
Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic l...
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Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...
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SoliD System chip-Seq Analysis The SOLiD System’s ability to generate over 400 million sequence tags (35 bp sequence reads) in a single run enables whole genome ChIP analysis of complex organisms. Sequence tags are mapped to a reference sequence and counted, to identify specific regions of protein binding. The ultra high throughput of the system provides researchers with the sensitivity and the...
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and negative control libraries are created, the samples are sequenced on the SOLiD System. The short sequence reads from the SOLiD System are mapped against genomic sequences using the SOLiD System alignment tools available through the Applied Biosystems software development community (http://info.appliedbiosystems. com/solidsoftwarecommunity/) or third-party tools compatible with SOLiD sequenc...
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Chromatin immunoprecipitation (ChIP) analysis is widely used to identify the locations in genomes occupied by transcription factors (TFs). The approach involves chemical cross-linking of DNA with associated proteins, fragmentation of chromatin by sonication or enzymatic digestion, immunoprecipitation of the fragments containing the protein of interest, and then PCR or hybridization analysis to ...
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ژورنال
عنوان ژورنال: Acta Biologica Hungarica
سال: 2016
ISSN: 0236-5383,1588-256X
DOI: 10.1556/018.67.2016.4.3